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macromolecular assemblies of repeating antibody-antigenantibody-antigen units are formed. This occurs at an optimum antigen/antibody ratio which is called the equivalence point. This complex is insoluble and will immunoprecipitate to form precipitin. In double immunodiffusion, a small amount (3 5 l) of antiserum is placed in a central well and a range of dilutions of antigen is added to similar wells surrounding it (Figure 5.27). Both antigen and antibody solutions freely diffuse through the agarose (hence double immunodiffusion). If there is speci c binding of antibody to the antigen, then precipitin forms. This is visible to the naked eye but may also be stained with coomassie blue after washing away nonprecipitated protein. If two different antigens (e.g. two different proteins) recognize the same antibody, a common precipitin line is formed, that is the two lines fuse (Figure 5.28). These proteins are said to have immunological identity. If the proteins are nonidentical, the precipitin lines cross. In the case of partial identity between the antigens, a spur is formed. This type of experiment gives useful evidence for structural similarity between different antigens. 5.6.2 Zone Electrophoresis/Immunodiffusion Immunoelectrophoresis In zone electrophoresis/immunodiffusion immunoelectrophoresis, it is rst necessary to perform electrophoretic separation of antigens in an agarose gel containing a trough as shown in Figure 5.29. After electrophoresis, a solution of antibody is poured into this trough. As with Ouchterloney double immunodiffusion, antigens and antibodies diffuse towards each other through the agarose and form precipitin arcs. This technique is useful for qualitative detection of antigens in serum, cell extracts or other preparations. 5.6.3 Rocket Immunoelectrophoresis

11. Double-click the Kerio Personal Firewall icon on your taskbar to open the main program window. 12. Click the Network Security tab, as shown in Figure 6-19, to view the current status of different programs and services on the Application tab. This tab lists whether programs are services are allowed inbound (In) or outbound (Out) access to your trusted network and the Internet. A green checkmark designates the action is always allowed; a yellow question mark means that you ll be prompted with an onscreen message when an attempt to perform that action occurs. A program or service that is denied access is marked with a red X icon. 13. By default, Kerio Personal Firewall configures itself such that you ll be prompted to make a decision when any program or service attempts inbound or outbound communication for the first time. To test this capability, click Start Internet Explorer and browse to a Web site. The Outgoing Connection Alert (Internet) box appears, as shown in Figure 6-20. 14. To allow Internet Explorer to always connect to the Internet, check the Create a rule for this communication and don t ask me again checkbox and then click Permit. This prevents Kerio Personal Firewall from alerting you the next time that Internet Explorer attempts to access the Internet.

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Some of these can be contained by a firewall, and others are difficult or impossible to filter using a firewall. E-mail spams get through the firewall. Antivirus software must be installed on each host.

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TABLE 7 Contents of Parts IA, IB, IC, ID, IE, and IF of Indian GMP Regulations Covering Speci c Guidance [24] Section Part IA Subject Speci c requirements for manufacture of sterile products, parenteral preparations (small-volume injectables and large-volume parenterals) and sterile ophthalmic preparations Speci c requirements for manufacture of oral solid dosage forms (tablets and capsules) Speci c requirements for manufacture of oral liquids (syrups, elixirs, emulsions, and suspensions) Speci c requirements for manufacture of topical products, i.e., external preparations (creams, ointments, pastes, emulsions, lotions, solutions, dusting powders, and identical products) Speci c requirements for manufacture of metered-dose inhalers (MDIs) Speci c requirements of premises, plant, and materials for manufacture of active pharmaceutical ingredients (bulk drugs)

Right now, most phishers assume that the user is browsing with Internet Explorer if they consider the matter at all Moreover, they tend to assume that the user is browsing with an unpatched version of Internet Explorer (Or the patch may not be out yet; it can be days, weeks, or months after a vulnerability is reported before Microsoft releases a patch) In December 2004, phishers were about 70% right: statistics reported that only 30% of XP users were running Windows XP Service Pack 2 In addition, I believe that the percentage of dial-up users running older versions of IE is much higher than the percentage of broadband users with older versions Service Pack 2 is 256MB When I was on dialup, I never managed to download anything that big, even if I left it overnight the connection would just fall over at some point.

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